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How Does Colormetric Analysis Work

By Melanie Vaughn


Colormetric analysis is actually a method of determining the concentration of a certain chemical element or compound in a solution. In order to do this successfully, a color reagent is used for identification purposes. This method is widely used in many laboratories for several purposes, but is commonly used for industrial purposes.

The equipment that is required to do the task is a colorimeter and those reagents that are suitable for the solution that will be tested. It can also be automated by the use of a machine that is especially made for this process. Though, one should be very careful when performing the process so that it will be a successful one.

You can only find the densities and concentrations of the solution after it is calibrated. The filter in the instrument must be set to red, especially if the solution is blue. The size of the filter is also very important so that the wavelength of light that is being transmitted must also be the same as that of those that are absorbed.

It has been observed that the organic reagent is more sensitive than those inorganic reagents. This is because of their stable nature, which makes them very selective to different processes that they undergo. Also, they are more able to give out intense kinds of colors, which are better sources of information to analyze.

So that the instruments can be used in the best possible way, there have to be different sets of solutions that must be used. The lengths of the path that the light can trace will then vary and shall be compared for the results. Also, there are some equations that must be used to get the equality of the components.

The enzymatic method of testing is also utilized by many analysts because it is easy and specific to use. They use a kind of enzyme in order to determine the concentration of a specific element. Also, it is done in an environment that is most suitable for the enzyme so that it will work on the process properly.

There are also others that make use of the effects of ultraviolet rays. However, there really is no visible changes in the color, when this process is used, but the principle used is the same all throughout the process. What they do is that they measure the absorption of color of each of the substances that are being involved.

There are also automatic colorimeter that have been developed. In the past, that make use a cuvette that is the one containing the control substance. Then, the light intensity is being calculated before it can pass through the instrument. Years after the analysts make use of it, new kinds of colorimeters are also invented.

Colormetric analysis can be considered a very helpful method in the laboratories that are being utilized by many industries. This is also very helpful in the industrial water treatment, which are also very important processes in many urban settings. This is a good way for determining the concentrations of different chemical elements.




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